| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_916075766043ce900399a4c2eee9f054 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-567
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
| filingDate |
2000-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2004-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90ba0d5c5f6c24ddf220f4da7a5b46da |
| publicationDate |
2004-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6824990-B1 |
| titleOfInvention |
Methods of detecting and modulating oligomerization of G protein-coupled receptors |
| abstract |
The present invention provides a method of detecting oligomerization of G protein coupled receptors comprising: a) obtaining a first G protein coupled receptor fusion protein containing a fluorescence donor; b) obtaining a second C protein coupled receptor fusion protein containing a fluorescence acceptor; c) transfecting a cell with the G protein coupled receptor fusion proteins of a) and b); d) exciting the fluorescence donor at a particular wavelength; e) detecting fluorescence emission of the acceptor (FRET), such that if this emission is greater than the emission detected in control cells expressing only the acceptor, oligomerization of the G protein coupled receptors has been detected. The invention further provides methods of detecting agonists and antagonists of G protein coupled receptors by utilizing FRET. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006223115-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019212946-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9217024-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006205015-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009018084-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006257770-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010125314-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7691564-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8697380-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011098309-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9006283-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8962677-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8647887-B2 |
| priorityDate |
1999-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |