http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6809816-B2

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
filingDate 2001-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2004-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9543de319e187d6c672c55a867806a70
publicationDate 2004-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6809816-B2
titleOfInvention Measurement of fluorescence decay times
abstract Laser pulses from laser head 12 pass through objective lens 16 to a focal spot in sample spot 17. If a pulse hits a fluorophore molecule in the focal spot a fluorescence photon is emitted which is collected by lens 16, reflected by dichroic mirror 15 through filter 20, which blocks photons of other wavelengths, to single photon counting photomultiplier unit 21. On detecting a photon, photomultiplier unit 21 generates an output pulse which is coupled, with a short delay, by pulse controller 30 to driver circuit 24 which causes laser head 12 to generate another laser pulse. Thus the interval between the laser pulses is substantially equal to the time between excitation of, and fluorescence emission by, the fluorophore molecule. Computer 23 coupled to photomultiplier unit 21 and driver circuit 24 determines the decay time by measurement of a number of fluorescence events.
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Total number of triples: 25.