| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-170769
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-177692 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2001-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2004-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faab021358ae62ab8cdb0d7562044981
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8afa5be1b7b87c8d1cbe0edab6dbbf8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f1edf56dfd5f2a338889f62e7d87e84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfd555af0c8927cba241465f5226eb74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32b32996a16d9c0f9578f96173b77d63 |
| publicationDate |
2004-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6806057-B2 |
| titleOfInvention |
Method for assaying protein nitrosylation |
| abstract |
Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. A method to detect proteins which contain nitrosothiols involves several steps. Nitrosylated cysteines are converted to tagged cysteines. Tagged proteins can then be detected, for example, by immunoblotting and/or can be purified by affinity chromatography. The method is applicable to the detection of S-nitrosylated proteins in cell lysates following in vitro S-nitrosylation, as well as to the detection of endogenous S-nitrosothiols in selected protein substrates. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006154376-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005238734-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9937144-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010266566-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006076225-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8679811-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8877447-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7507585-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006076225-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8053243-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008014595-A1 |
| priorityDate |
2000-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |