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filingDate 2001-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2004-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_723dca01629561652fe95bd9e4577a03
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publicationDate 2004-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6759231-B2
titleOfInvention Phage with nuclear localization signal
abstract A lambda phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a lambda phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant lambda phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting lambda phage is capable of packaging lambda phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.
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