http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6703200-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22b8ba6cc302f580e5af24ab2fff50f1 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01M4-663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E60-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E60-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02T10-70 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01G11-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01G11-44 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01M4-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01G9-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01G9-00 |
| filingDate | 2000-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96352081e73bc336dc7307faebd46dbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e18e8bf93d4364665c7bda252c4a5fa |
| publicationDate | 2004-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-6703200-B1 |
| titleOfInvention | Methods and materials for the rapid and high volume production of a gene knock-out library in an organism |
| abstract | The present invention relates to a method for facilitating site directed homologous recombination in an organism to produce mutants comprising:1) providing a large insert vector library comprising one or more large insert vectors, each of said large insert vectors comprising a piece of DNA, said DNA piece comprising multiple genes from a target organism and a first selectable marker functional for selection in bacteria;2) providing a second vector comprising a transposable element, said transposable element comprising a nucleotide sequence coding for a second selectable marker flanked on each side by an inverted repeat sequence, wherein said selectable marker is bifunctional for selection in bacteria and the target organism and wherein said inverted repeat sequences are functional as a binding site for a transposase;3) incubating said library with said second vector in the presence of a transposase specific for the inverted repeat sequences on the plasmid vector, such that the transposable element is transferred randomly into an individual large insert of the large insert library to produce disrupted large insert vectors;4) optionally, amplifying the disrupted large insert vectors resulting from step 3);5) introducing at least one of said disrupted large insert vectors into a target host cell; and6) selecting for successful homologous recombination in said target host cell using the second selectable marker. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109467594-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109467594-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006172379-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9045748-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005121351-A3 |
| priorityDate | 1999-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 717.