Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb8f3eba86915d967beefc175437273a |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2799-021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-61 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
filingDate |
1998-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2003-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49aeb7bd16d4a30080526d4ef82720ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7196e69a19ac98fcb8b978a6be05d007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e6c4062148dc8bab451140ff0be76f6 |
publicationDate |
2003-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6670449-B1 |
titleOfInvention |
Hybrid molecules and their use for optically detecting changes in cellular microenvironments |
abstract |
The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7977097-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113501881-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014315820-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113501881-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10517557-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11078519-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003212265-A1 |
priorityDate |
1997-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |