| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b828305495e1cdb63947a3a18aa633d1 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate |
2001-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2003-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c7e542c080147d1a94ac6bf893770bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb50f30a48fd23145c8584a9f05c949b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91e760c12cf10ecdd2f92b7c77133d9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bed44ed41c4953445849c59381e277f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3277f4442fa26d6bbb9fb522bd3c091e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2397be9d54df4b3cb7027e949b30b6af |
| publicationDate |
2003-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6646185-B2 |
| titleOfInvention |
Genetic transformation method for zoysiagrass |
| abstract |
The present invention provides an efficient genetic transformation system for the zoysiagrass plant (Zoysia japonica Steud.). Also provided are optimized media compositions and culture conditions for the zoysiagrass transformation. The reliable transformation system for zoysiagrass was developed by optimizing several factors that significantly affect calli growth and plant regeneration. Callus type and co-cultivation period absolutely influenced the transformation efficiency. Concentrations of 2,4-D, CaCl2 and acetosyringone were also critical factors. The best result was achieved when type 3 calli were co-cultivated on a 2,4-D-free co-cultivation medium for 6 days. Removal of calcium and addition of 50 mg/liter acetosyringone during co-cultivation drastically enhanced the efficiency. The invention also provides a bialaphos-resistant zoysiagrass plant, which can be used in golf courses and athletic fields to save the maintenance cost. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006101536-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013005211-A2 |
| priorityDate |
2001-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |