| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d98ee604a6d8717f0b0c75c288cee56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_424db9d56b06a23aed410fcf5df652f3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-006 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
| filingDate |
2001-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2003-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_814a2c6999fd265b67971360309f8730
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4465bef300199a8fa4953799c41f2a06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d5592d92f3a90b0b5a0410dc0d88d9c |
| publicationDate |
2003-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6602997-B2 |
| titleOfInvention |
Whole cell and cell-debris polysaccharide |
| abstract |
The present invention provides for a method for obtaining a polysaccharide substantially free from whole bacterial cells and bacterial cell debris including:(a) fermenting a Sphingomonas bacterium, which produces the polysaccharide, to produce an aqueous fermentation broth having the polysaccharide dissolved therein;(b) diluting the fermentation broth with an equal volume of deionized water;(c) partially hydrolyzing the polysaccharide by exposing the fermentation broth resulting from step (b) to a temperature in excess of 100° C. for a time period from about ten minutes to about one hour;(d) removing bacterial cells from the polysaccharide by centrifugation of the fermentation broth and recovery of a supernatant aqueous liquid;(e) precipitating the polysaccharide from the supernatant aqueous liquid by adding a second liquid to the supernatant aqueous liquid, wherein the second liquid is miscible with water, non-reactive with the polysaccharide, and in which the polysaccharide is substantially insoluble; and(f) harvesting the polysaccharide resulting from step (e) by separating it from the supernatant aqueous liquid. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2422807-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2286840-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2286838-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2286837-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2330124-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2392595-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2417980-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2390264-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2330125-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2264064-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2127676-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-1995326-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3524261-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2347762-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2422806-A2 |
| priorityDate |
2000-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |