| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-824
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-826
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2730-10122 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate |
1988-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2003-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7db3908571701dc5c874fcc967cbf957
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_304eef6343ee638ce2fecd5755e325f7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d71e9402ff948eed5a3c88eba2a4fa9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5e7c2d8ee2cf3ba8fe646f6b7a12297 |
| publicationDate |
2003-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6544757-B1 |
| titleOfInvention |
Synthesis of human virus antigens by yeast |
| abstract |
The present invention relates to synthesis of HBSAg in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBSAg by yeast has been achieved. The product is antigenic (reactive with anti-HBSAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBSAg synthesized by yeast has identical sedimentation behavior to purified, naturally occurring HBSAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9452212-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9884110-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10314907-B2 |
| priorityDate |
1981-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |