| abstract |
The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein.These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector. |