| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_750cdbf52fb47f5fe567c5d8e02f47e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79deab07c6ddf5e785dc808dafef38b4 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
1998-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2003-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_691a6be5e1b03f35d0c4be2c38dfcdca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_222e88964407f2e93fd9e7518400ed4c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42264921d569774beb8d17578b456b9d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34b7d6433bba7b7b273f83db1f1f22d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18fdb7e5923fee3b42025310033af26c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32fae1f11335af61cd4bb45df6571fa7 |
| publicationDate |
2003-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6544736-B1 |
| titleOfInvention |
Method for synthesizing cDNA from mRNA sample |
| abstract |
cDNA including the 5'-terminal sequence of full-length mRNA with a cap structure is synthesized from a mRNA sample containing the full-length mRNA with the cap structure and non-full-length mRNA without any cap structure in mixture. At the first step, the phosphate group at 5'-terminus of the non-full-length mRNA in the mRNA sample is removed. At the second step, the cap structure at the 5'-terminus of the full-length mRNA in the mRNA sample is removed. At the third step, an oligoribonucleotide is ligated to the phosphate group at 5'-terminus of mRNA generated through the first and second steps. At the fourth step, mRNA with the oligoribonucleotide ligated at the 5'-terminus thereof at the third step is subjected to a reverse transcriptase process using a short-chain oligonucleotide capable of being annealed to an intermediate sequence within the mRNA as primer, to synthesize a first-strand cDNA. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284549-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004006030-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE48909-E
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011014623-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7833716-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8580510-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10167500-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010062946-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8034570-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8278052-B2 |
| priorityDate |
1997-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |