Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60da379fb33c192ef77f86a60c5ac029 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
2001-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2003-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1ac633cee523619e424b56c098f8157 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41e57b36406348fd4474d9c8a159161c |
publicationDate |
2003-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6528295-B2 |
titleOfInvention |
Protein phosphatase methylesterase |
abstract |
Carboxymethylation of proteins is a highly conserved means of regulation in eukaryotic cells. The protein phosphatase 2A (PP2A) catalytic (C) subunit is reversibly methylated at its carboxy-terminus by specific methylesterase. Carboxymethylation affects PP2A activity and varies during the cell cycle. The present disclosure provides the coding sequence of a methylesterase, herein named Protein Phosphatase Methylesterase-1 (PME-1). PME-1 is highly conserved from yeast to human and contains a motif found in lipases, which motif has a catalytic triad-activated serine as the active site nucleophile. Recombinant PME-1 polypeptide produced in bacteria demethylates PP2A C subunit in vitro and okadaic acid, a known inhibitor of the PP2A methylesterase, inhibited this reaction. PME-1 represents the first mammalian protein phosphatase methylesterase cloned to date. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006171938-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7923041-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8221804-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007122880-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9486441-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10583119-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7794965-B2 |
priorityDate |
1998-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |