http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6500619-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a3f1a6ecdf078fea0c5b6db10127883 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate | 2000-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3334ef348a7fc8fb37b5ab4936d71d0e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a3fe2561fbd838b082702dfba1d36e7 |
| publicationDate | 2002-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-6500619-B1 |
| titleOfInvention | Method for making an improved cloning vector containing marker inactivation system |
| abstract | Provided are lacZalpha gene fragments which have been modified to introduce multiple restriction enzyme sites. Vectors according to the present invention include at least one promoter operatively linked to a DNA sequence encoding lacZalpha(alpha-peptide); multiple cloning sites cleavable by distinct restriction enzymes which have been introduced within a lacZ coding sequence from and including the codon for amino acid 8, and in the lacZ coding sequence downstream of the codon for amino acid 8, in forming the modified lacZalpha coding sequence; and a replicon. Also provided are methods of using the vectors wherein a DNA molecule is cloned into at least one restriction enzyme site in the modified lacZalpha coding sequence, in forming recombinant vectors; introducing the recombinant vectors into competent host cells; growing the host cells in the presence of a chromogenic substrate cleavable by beta-galactosidase; and screening for indicia of lac operon marker inactivation. The invention also provides a method for identifying insertional inactivation sites within the coding sequence of a cloning vector that results in lower false negatives. |
| priorityDate | 1997-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 601.