| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69108fc12b940ede105638f37f06080b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 |
| filingDate |
1998-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2002-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77ca422f9695ba47f3c96203a15a5d3d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91927c3733fb6de94a51a2789c3e6213
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0833a6f534e3bfe1009b9a9b0cc84c36 |
| publicationDate |
2002-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6489162-B1 |
| titleOfInvention |
Helper-free stocks of recombinant adeno-associated virus vectors |
| abstract |
The present invention relates to a method for producing helper-free stocks of recombinant adeno-associated virus (rAAV) which can be used to efficiently and stably transduce foreign genes into host cells or organisms. The method comprises the cotransfection of eukaryotic cells with rAAV and with helper AAV DNA in the presence of helper virus (e.g. adenovirus or herpesvirus) such that the helper AAV DNA is not associated with virion formation. The crux of the invention lies in the inubility of the helper AAV DNA to recombine with rAAV vector, thereby preventing the generation of wild-type virus. In a specific embodiment of the invention, the vector comprises a recombinant AAV genome containing only the terminal regions of the AAV chromosome bracketing a non-viral gene, and the helper AAV DNA comprises a recombinant AAV genome containing that part of the AAV genome which is not present in the vector, and in which the AAV terminal regions are replaced by adenovirus sequences. In a further embodiment of the invention, cell lines are created which incorporate helper AAV DNA which can directly produce substantially pure recombinant AAV virus. The pure stocks of recombinant AAV produced according to the invention provide an AAV viral expression vector system with increased yield of recombinant virus, improved efficiency, higher definition, and greater safety than presently used systems. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9506052-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6927281-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2484780-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11013774-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010284971-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8906387-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009191597-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8198421-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018013705-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8632765-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8574583-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005287662-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009214496-A1 |
| priorityDate |
1989-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |