| abstract |
Genomic DNA and cDNA encoding DELTA9-desaturase from a microorganism belonging to the subgenus Mortierella of the genus Mortierella, an expression vector for expression thereof, and a transformant are disclosed. A method for producing DELTA9-desaturase by use of a gene encoding the enzyme is also disclosed. Introduction of the DELTA9-desaturase gene of the present invention into an unsaturated fatty acid producing cell can enhance conversion into palmitoleic acid or oleic acid, starting materials for unsaturated fatty acids, and can increase the production of unsaturated fatty acids. By combining a gene for cytochrome b5 or a gene for cytochrome b5 reductase, constituents of the microsomal electron transport system, with the DELTA9-desaturase of the present invention, more efficient production can be expected. DELTA9-desaturase can also be produced with high efficiency by a recombinant DNA technology. |