abstract |
The invention relates to a method for determining homocysteine concentration in samples in which homocysteine is condensed using an enzyme cystathionine β sythase to form cystathionine. Pyruvate and/or ammonia are released from cystathionine by action of an enzyme, cystathionine β lyase, and homocysteine is regenerated. The release of pyruvate and/or ammonia can be correlated to the concentration of homocysteine present in the sample. |