| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_588afa6226c42bef05e3e02cd623ee4e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P13-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-08 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P43-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P13-02 |
| filingDate |
2000-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_213b494f08a56e3870155a6ce79756d8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30e0b1a6ea9fc236e1f4873bb63269b2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74304015f29e436c9816fb44f6b5abbf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fa7cf800e49cd27cc08a395a9e0ba30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_526ce6da38ac24713fdeed1e96a6c02b |
| publicationDate |
2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6403361-B1 |
| titleOfInvention |
Enzymatic method for modification of recombinant polypeptides |
| abstract |
The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit. The invention utilizes known and newly discovered cleavage recognition sites to effectuate the desired modification products. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6849708-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6551693-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7544512-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7138486-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005119178-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004146985-A1 |
| priorityDate |
1993-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |