Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45753cecc639997fe19d195ad3d6a74a |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
1999-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4563c11db2d8424ad277f018181c776 |
publicationDate |
2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6403339-B1 |
titleOfInvention |
Method for detecting a nucleotide sequence |
abstract |
A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5'-terminus of a central segment. A second primer is added and is complementary to a 5'-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5'-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008241890-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11209368-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9353405-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004092412-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9261460-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8088576-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009269737-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10144957-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005137388-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007219366-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004020603-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004020603-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009081650-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9316587-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004171163-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7993828-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7244566-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006000647-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004224319-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005106023-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9068948-B2 |
priorityDate |
1998-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |