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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 1999-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4563c11db2d8424ad277f018181c776
publicationDate 2002-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6403339-B1
titleOfInvention Method for detecting a nucleotide sequence
abstract A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5'-terminus of a central segment. A second primer is added and is complementary to a 5'-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5'-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel.
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