http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6369207-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6867 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1999-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3909e4deca2c1bdbf666a562878a5028 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea82d255ce961edc20e8ebe99aa59894 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71617b1082da1912c4b1b0cc233c682c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c31927e3e0aefda30051879d2854ca93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_913ebeac7a5cec29bdb6c0ae36794597 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28c9f11aed1a60bf87acb5e77b1295fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3f6b89575b755d8e3ef94e1a2403b45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3c40d1ef752281d925abdc279a150ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e375cad1f6c48d611933d8c078429d70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eed4d4de943411d5ccc5f2ec9625d379 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f959bd1ee2303c8dc1af7bc56ea89e4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff0bf496f9f8e002781a520e0a30f5d0 |
| publicationDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-6369207-B1 |
| titleOfInvention | Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases |
| abstract | The present invention entails methods, and kits for carrying them out, based on the discovery that an RNA replicase, such as Qbeta replicase, has DNA-dependent RNA polymerase ("DDRP") activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2'-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase. The discovery of this DDRP activity provides methods of the invention for nucleic acid amplification wherein a nucleic acid, with a DNA segment with the sequence of an RNA that is autocatalytically replicatable by an RNA replicase, is provided as a substrate for the replicase. The replicase catalyzes synthesis, from the DNA segment, of the RNA, which the replicase then autocatalytically replicates. The invention entails use of the amplification methods in detecting nucleic acid analytes, as in nucleic acid probe hybridization assays. Such assays of the invention include those wherein a nucleic acid analyte is hybridized with one or more nucleic acid probes, which include or are processed to generate a DNA segment which is amplifiable through production from the segment, catalyzed by the DDRP activity of an RNA replicase, of an autocatalytically replicatable RNA, which is autocatalytically replicated to provide an abundance of readily detectable reporter molecules. The invention permits replacement of an RNA, that is autocatalytically replicatable with an RNA replicase and employed as a reporter or label in prior art assays, such as nucleic acid probe hybridization assays or immunoassays, with a nucleic acid comprising a DNA segment with the same base sequence as the RNA. The invention also includes the methods of the invention with Mn+2, Co+2, or Zn+2 in the solutions in which the DDRP activity occurs. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004059005-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007122804-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004059005-A3 |
| priorityDate | 1990-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 121.