http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6368815-B1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_05ff905fdc49089f5f25746eaeb40190 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5038 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-502 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5023 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
filingDate | 2000-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49652e0035c6366dce9975dbc2a32bdf |
publicationDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-6368815-B1 |
titleOfInvention | Screening of molecules that inhibit human phosphodiesterase 4A produced by non-recombinant cell lines |
abstract | An assay for the screening of a candiadte molecule that inhibits the expression or the activity of a human phosphodiesterase 4A (PDE4A) is disclosed. The method comprises incubating non-recombinant cells in the presence of a concentration of a phorbol ester sufficient to significantly increase PDE4A production in the cells. Next, a candidate molecule is added to the culture medium in which the phorbol ester-treated cells are cultured, then the cells are disrupted and harvested to produce a cell lysate. Finally, the cAMP content in the cell lysate is quantified and then the cAMP content in the cell lysate is compared to the cCAMP content in the cell lysate of the phorbol ester treated cells cultured in the absence of the candidate molecule. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003220273-A1 |
priorityDate | 1999-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 366.