| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_634d7355c2673489b2f4f800541770bb |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0271
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate |
2000-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2002-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_293218120cf40c7256e1def84ccc89f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_552745441769d4587297a6c93a9eb4df
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51b2276a67721520b3660cb22e9a1632 |
| publicationDate |
2002-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6344324-B2 |
| titleOfInvention |
Quantification of cellular injury using expression of a fluorescent protein under the control of the GADD153 promoter |
| abstract |
The present invention features a novel cellular injury reporter system in which a chimeric gene containing the GADD153 promoter linked to the coding region of an enhanced green fluorescent protein (EGFP) gene was stably integrated into the genome of carcinoma cells. Activation of the GADD153 promoter was quantified using flow cytometric measurement of EGFP expression following drug exposure. This reporter system is suitable for high. throughput in vitro and in vivo screening for agents capable of producing cytotoxicity via a wide variety of different mechanisms, and can be utilized to investigate the relative potency of structurally related DNA adducts. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7306905-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9285358-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02095061-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011105968-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011070059-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100655792-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003008272-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012137186-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103582704-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005221324-A1 |
| priorityDate |
2000-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |