| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a06295826f92f3cf3ab6a78cbd2fdd19 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6818
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
1998-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2001-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f02ee98e4f3b8d0606d68646a2b9421
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2314d5c34f5afbf10042aad8ed40403 |
| publicationDate |
2001-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6294333-B1 |
| titleOfInvention |
Fluorescent intensity assay for protein or peptide binding to nucleic acids |
| abstract |
A method for assaying binding between a fluorophore-labeled compound and an unlabeled compound is provided. The method includes detecting a quenching effect on fluorescence emitted by the fluorophore-labeled compound resulting from binding. The binding is specific and other than nucleobase to nucleobase. The method is conducted without separating complexes of the fluorophore-labeled compound and the unlabeled compound from the fluorophore-labeled compound prior to quenching effect detecting, and without providing a signal quenching agent to quench fluorescent light. Preferably, the fluorophore-labeled compound is a nucleic acid and the unlabeled compound is a protein. The method can be used for a variety of applications, including screening for drug candidates having optimum binding properties, and quantifying the binding affinity of DNA binding proteins for nucleic acids. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6613524-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004180345-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6982147-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8486622-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002123066-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006019282-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6858390-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009123914-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010197041-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003170659-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003049673-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7052844-B2 |
| priorityDate |
1998-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |