| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53877e6bc8dc204b647d8908c4292cc1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-808
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-975
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5091
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate |
1998-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2001-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a790c564453a9c8c36a7303e06ca693
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61ea93ea2007de6eb2afae23f1c2aa7e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb1d034663c69f04d06385bc768c827e |
| publicationDate |
2001-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6277655-B1 |
| titleOfInvention |
Assay and reagent kit for evaluation of multi-drug resistance in cells |
| abstract |
Disclosed herein are methods and reagents kits forth quantitative in vitro determination of the functional determination of multi-drug resistance in cells, as well as for the clinical screening of potential modulators of multi-drug resistant transport activity in cells. The method of the invention is based on the measurement of the accumulation rate of free calcein within the cells of the specimen (advantageously by flourescence measurement), after exposing the cells in vitro to a cell permeable form of calcein that is a good substrate for MDR proteins present in the sample. The cell permeable form of calcein is converted within the cell by intracellular enzymes to free calcein. Comparison of free calcein accumulation in the presence and absence of a potential inhibitor of transport activity permits the rapid screening of such inhibitors. Additionally, evaluation of free calcein accumulation rates in the presence of inhibitors of all MDR transport in comparison with calcein accumulation rates in the presence of selective inhibitors of MDR transport permits the evaluation of the functional type of MDR being exhibited. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11726039-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005282244-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005116648-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008206781-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8748159-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1899728-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1899728-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3272852-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10684276-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7527924-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005116648-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6665430-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003207327-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11789013-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009104652-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004265323-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007009533-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007009535-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007010478-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007010466-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7906295-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8071326-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6459805-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7838256-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9880153-B2 |
| priorityDate |
1994-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |