| abstract |
Propargylethoxyamino nucleosides are disclosed having the structurewherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides. |