abstract |
A method of culturing bovine embryos by culturing said bovine embryos in a medium containing thiol compound, and also a method of transporting bovine embryos by transporting said bovine embryos in a medium containing thiol compound are disclosed.According to the present invention, it is possible to culture bovine embryos up to blastocyst stage efficiently in the medium containing low molecular thiol compound without in vivo culture or coculture using feeder cells. Also when low molecular thiol compound is added to the transportation medium for bovine embryos, almost 100% embryos are still viable after long distant transportation. |