Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c4322cb4de342be2dbedcbe0c278f6f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-32 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4722 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-39 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
filingDate |
1998-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2001-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d87c9192dffa31e0a735f6d5143b33be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90ddbf7003d1045f61d5c2c15be9226c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3503d0998dd693de9c52896d918a9632 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c7455885754039a7914efd8897caa95 |
publicationDate |
2001-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6168927-B1 |
titleOfInvention |
Expression of G protein coupled receptors in yeast |
abstract |
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein alpha subunit (mammalian Galpha). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein alpha-subunit (yeast Galpha) The cells are useful for screening compounds which affect the rate of dissociation of Galpha from Gbetagamma in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11092599-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1868634-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1868634-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8748480-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2001026926-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7122305-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11009502-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7105309-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11460469-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003203417-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006275828-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10209239-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7235648-B1 |
priorityDate |
1990-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |