| abstract |
PCT No. PCT/GB94/00849 Sec. 371 Date Jan. 25, 1996 Sec. 102(e) Date Jan. 25, 1996 PCT Filed Apr. 21, 1994 PCT Pub. No. WO94/24307 PCT Pub. Date Oct. 27, 1994This invention relates to DNA constructs for inserting heterologous gene sequences into a host genome so as to obtain expression of the heterologous gene, to methods of inserting heterologous gene sequences into a host genome, and to organisms carrying modified host genomes. Specifically, the DNA constructs of this invention contain an expression unit of an internal ribosome binding site (IRES) coupled to a heterologous gene sequence. This expression unit is flanked at the 5' and 3' ends by DNA sequences that enable homologous recombination or integration of the construct with the DNA of a targeted host to obtain expression of the heterologous gene in the host. |