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filingDate 1999-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2000-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6129919-A
titleOfInvention Method of producing fermented sword beans
abstract A method of producing fermented sword beans to obtain composition of the sword bean that has inhibition action on cancer cells. The method includes steps of selecting proper sword beans and soaking the sword beans in an adequate amount of water, disinfecting the sword beans under high pressure and high temperature of 105° C. for one hour, letting the sword beans cool to 38° C., well mixing the sword beans with Aspergillus oryzae of 1.5/1000% in concentration, allowing the mixture to ferment in three separated stages for total 80 hours, adding water of five times in volume of the fermented mixture and well mixing them, extracting to obtain clear solution of the fermented sword beans, and freeze-drying the solution into powder. Tests conducted in laboratory indicate that liver cancer cells, HepG2, have a viability dropped by 80% when treated with the fermented sword bean solution of 200 μg/ml in concentration, and zero when treated with the same solution of 400 μg/ml in concentration, while the viability of normal liver cell line, Chang, is only affected by about 10%.
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