| abstract |
A method for producing glycoconjugate, which comprises the steps of: n (i) binding a sugar residue to the side chain of a water-soluble polymer via a linker having a selectively cleavable linkage to give a primer, and bringing said primer into contact with an immobilized glycosyltransferase in the presence of a sugar nucleotide, to transfer a sugar residue of said sugar nucleotide to the sugar residue of said primer, n (ii) elongating a sugar chain by transfer of plural sugar residues by repeating the step (i) at least once, n (iii) removing, where necessary, a by-produced nucleotide or an unreacted sugar nucleotide, and n (iv) repeating the steps (i)-(iii) where necessary and releasing the glycoconjugate by selectively cleaving the cleavable linkage in the linker, from the above-mentioned primer connecting the sugar chain elongated by the transfer of plural sugar residues, n and a method for producing a sphingoglycolipid. |