| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97ddd4d1d0ebc8a7d2c8e7d214d08cf3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate |
1995-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2000-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7064a4a139bcbabb7fd5a89e88c7e64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb9b59f5b526d3a330fd4870c4e788e0 |
| publicationDate |
2000-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6037145-A |
| titleOfInvention |
Process for production of protein |
| abstract |
A process for the production of a desired polypeptide comprising the steps of: (1) transforming host cells with an expression vector comprising a gene coding for a fusion protein comprising a desired polypeptide and a protective polypeptide; (2) culturing the transformed host cells so as to express said gene to produce a fusion protein; and (3) excising the desired polypeptide from the fusion protein with a protease intrinsic to the host cells. According to the present invention, a large amount of a desired polypeptide can be produced at a low cost. Especially according to the present invention, a large amount of S. aureus V8 protease can be efficiently produced at low cost using a safe host such as E. coli according to gene recombination procedures. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7794979-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113004419-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009048428-A1
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006074009-A1
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008207505-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7732569-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115182-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009015165-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016109872-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010234568-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009043075-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008206809-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7795382-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7378078-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010186633-A1 |
| priorityDate |
1994-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |