| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21069
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6459
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8114 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1997-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1999-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca7f807ddde5d044f75254d3af0cb3ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c218274af4acd35eb0a56936e02373b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53550a7a66ab0ef8ce23e017f9a2f507
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa111d37d514e21ca13528e9a441d160 |
| publicationDate |
1999-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5973118-A |
| titleOfInvention |
Mutant of the Erythrina caffra type inhibitor and the use of the said mutant for purifying serine proteases |
| abstract |
A polypeptide which has the activity of an inhibitor DE-3 from Erythrina caffra and which reversibly and selectively binds serine proteases from a protein mixture is obtainable by culturing prokaryotic or eukaryotic host cells which have been transformed or transfected with a nucleic acid that codes for the said polypeptide in a manner that allows the host cells to express the said polypeptide under suitable nutrient conditions and isolating the said polypeptide, wherein the polypeptide has an amino acid sequence which is functionally analogous to SEQ ID NO:2, has a partial region that is more than 85% homologous to the region of amino acids 39-139 of this sequence, has two disulfide bridges and begins N-terminally with SEQ ID NO:4 or with a SEQ ID NO:4 extended N-terminally by methionine and has a binding capacity for tissue plasminogen activators of 1.25 MU/ml and more and is particularly suitable for purifying plasminogen activators such as tissue plasminogen activators (t-PA and derivatives). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6770457-B1 |
| priorityDate |
1995-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |