| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-82 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61 |
| filingDate |
1997-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1999-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c92df18c9d3cd1c8f4a6ee8f657fb4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7afb496c1492f3969da1ea3fa16dcef6 |
| publicationDate |
1999-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5968767-A |
| titleOfInvention |
Methods for in vitro protein synthesis |
| abstract |
A method is provided for in vitro protein synthesis in a bacterial extract wherein the reaction mixture comprises a reducing agent and dissolved oxygen (DO2) wherein the DO2 concentration is regulated such that complete oxidation of the reducing agent concentration does not occur for a period of at least about 30 minutes following initiation of protein synthesis in the reaction mixture. Also provided is a method for in vitro protein synthesis in bacterial extracts wherein the reaction mixture comprises an initial methionine concentration of at least about 1.0 mM, or wherein the reaction mixture comprises both labeled and unlabeled methionine, and the initial concentration of unlabeled methionine is at least about 0.1 mM. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7338780-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1553184-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9175327-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1848812-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006039622-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10774304-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004219631-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7749742-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6242189-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012231496-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1553184-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016222341-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9744175-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11702625-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9982227-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10435664-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1848812-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9617533-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9896483-B2 |
| priorityDate |
1996-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |