| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97ddd4d1d0ebc8a7d2c8e7d214d08cf3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate |
1997-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1999-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80c96d5dbfa4d8599a46c3a1ce845380
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0ae167e33eacca8997f40c1af218d3a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d68b282cfabf595fa9daac0d87e3052
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78a782ff61e48644d7f49e8d6a7b62be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e84c3edff2663526d251706144215d8 |
| publicationDate |
1999-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5965444-A |
| titleOfInvention |
Method for constructing transformant not having selective marker gene |
| abstract |
A DNA construct wherein a DNA fragment which is recombinable in yeast chromosomal DNA is directly or indirectly linked at both ends of a DNA fragment which comprises a pair of R sensitive sequences oriented in the same direction and flaking both an R gene placed under the control of an inducible promoter and an expressible selective marker gene, which is a DNA construct designed with the R sensitive sequences non-symmetrically shortened, so that no functionable R sensitive sequence remains after the R sensitive sequence recombination has occurred by expression of the R gene and the selective marker has been removed. Since no functionable R sensitive sequence remains after removal of the selective marker, recombination does not occur again, and thus the same selective marker may be used for multiple insertions of foreign genes. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011183387-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7696335-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007087366-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011053219-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8597931-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019201276-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6723562-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8748129-B2 |
| priorityDate |
1996-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |