abstract |
PCT No. PCT/AU95/00536 Sec. 371 Date May 14, 1997 Sec. 102(e) Date May 14, 1997 PCT Filed Aug. 25, 1995 PCT Pub. No. WO96/06185 PCT Pub. Date Feb. 29, 1996The present invention relates to a method of detecting the presence of an active protease in a sample. The method involves the following steps: (1) providing a solid support to which is attached a ligand reactive with a protease; (2) contacting the solid support with a sample suspected to contain the protease; (3) washing the solid support to remove material not bound to the ligand; (4) contacting the washed solid support with a solution containing a peptide ester substrate which is hydrolysed for the protease, and (5) detecting protons produced by hydrolysis of the peptide ester substrate. In addition, the present invention provide a prostate specific antigen substrate, the substrate being of the general formula; A-B-Tyrosine-ester in which A is acetyl or carbobenzoxy and B is 2 to 6 amino acids. |