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filingDate 1997-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1999-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5908787-A
titleOfInvention Total protein detection method
abstract Disclosed is an improved assay for the determination of total protein in an aqueous test fluid such as urine. The assay involves combining the test fluid with a molybdate or tungstate salt and a dye which forms a complex with molybdate or tungstate ion at a pH of 1.0 to 3.0 to shift the absorption band of the complex in the presence of protein. There is introduced into the assay a substituted phenolsulfonephthalein dye having a pKa which enables it to operate at a pH of from about 1.0 to 3.0 and whose affinity for protein is such that it will provide a detectable response only in the presence of greater than 50 mg/dL of protein to increase the scope of the assay.
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