http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5906928-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45a3703d03a4b21be619937f5ad63b8b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-13043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 1994-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1999-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e945f237c031483c56a936d238e5a825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b350db1c47afde02b947e1d48b80366 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3bf3812efcfe5f1b13a79930b88ba22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7fc58fcc132f88c08b764aa06033c95 |
| publicationDate | 1999-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-5906928-A |
| titleOfInvention | Efficient gene transfer into primary murine lymphocytes obviating the need for drug selection |
| abstract | PCT No. PCT/US94/08612 Sec. 371 Date Mar. 19, 1996 Sec. 102(e) Date Mar. 19, 1996 PCT Filed Aug. 1, 1994 PCT Pub. No. WO95/07358 PCT Pub. Date Mar. 16, 1995The present invention pertains to a method for efficiently introducing exogenous genes into primary lymphoid cells without drug selection which comprises the steps (a) deriving a retroviral vector and a helper cell combination that will yield a level of virus production in the range from 5x106 to 5x107 units/ml by transfecting a vector into a helper cell followed by selection, isolation of cell clones, and determination of viral titers to identify which virus-producing cell lines produce a virus titer in the range from 5x106 to 5x107 units/ml; (b) isolating a lymphoid cell subpopulation which can repopulate a specific lymphoid lineage or is a long-lived population by treating a suspension of lymphoid cells with a monoclonal antibody which removes undesired lymphoid cells to obtain an enriched lymphoid subpopulation; (c) culturing the enriched lymphoid subpopulation from step (b) with growth factors specific to the lymphoid subpopulation; (d) co-cultivating the lymphoid subpopulation from step (c) with a lawn of irradiated virus-producing cell line from step (a) to produce an infected lymphoid subpopulation; and (e) harvesting the infected lymphoid subpopulation. The invention further relates to a population of transfected lymphocytes, in which greater than about 90% of the lymphocytes contain a provirus. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8133473-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004022737-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106434551-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8715743-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7897140-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7097826-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7078014-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006204437-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6534040-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9709574-B2 |
| priorityDate | 1993-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.