| abstract |
PCT No. PCT/SE94/01023 Sec. 371 Date Apr. 15, 1996 Sec. 102(e) Date Apr. 15, 1996 PCT Filed Nov. 1, 1994 PCT Pub. No. WO95/12663 PCT Pub. Date May 11, 1995The invention relates to a method of cultivating mammalian cells expressing recombinant Factor VIII in a serum-free cell culture medium of the type that normally requires the presence of human serum albumin (HSA) and which is substantially free from fatty acids, fattyacid esters and lipids, but wherein HSA is replaced by at least one glucose or sucrose based polysaccharide having an average molecular weight of from about 10,000 to about 450,000. The invention also relates to such a serum-free cell culture medium. |