| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1311
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-745
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0643
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-502
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5088
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-745 |
| filingDate |
1997-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1999-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da1a229d4d221f5cc3743e69b6605f80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3c4c53829db2aa384e3545748739d74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4bcbeb21ad16257c29d5a9dab07a941 |
| publicationDate |
1999-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5856186-A |
| titleOfInvention |
Method for producing a highly enriched population of osteoclast cells |
| abstract |
Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a alpha v beta 3 receptor ligand, such as echistatin is used for cell detachment. The result is an 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP+) cells, in high yields (2-3x106 cells per experiment) can be obtained. These cells are mostly mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009104203-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007130619-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0112785-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6093533-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8101564-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7087431-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6428973-B1 |
| priorityDate |
1995-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |