| abstract |
The present invention is directed to methods for producing naturally secreted human extracellular matrix material and compositions containing the extracellular matrix material. The method includes culturing extracellular matrix-secreting human stromal cells on a biocompatible three-dimensional framework in vitro. After secretion of the extracellular matrix onto the framework, the stromal cells are killed and the cells and cellular contents are removed from the framework. The extracellular matrix material deposited on the framework is collected and further processed to obtain a physiologically acceptable compositions. The compositions of the present invention are useful for the repair of soft tissue and skin defects, including wrinkles and scars. |