http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5808009-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_107ea2b8f946a3d2e1a7edf0e96121db |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4354 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 1996-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1998-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ebf5a3057395a45d443c436fe14a74a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_702b0ced63b2344da40e5bb23990111c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf49b80da54dbd69b69bbad8a95cfdce |
| publicationDate | 1998-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-5808009-A |
| titleOfInvention | Method of affinity purifying antibodies |
| abstract | The present invention relates to a method of affinity purifying antibodies consisting of the following steps: A) homogenizing Helminth parasite worms forming a mixture; B) incubating the mixture of adult Helminth parasite worm homogenate with pre-swelled CNBr-activated Sepharose 4B in coupling buffer; C) first washing the mixture with blocking buffer; D) second washing the mixture with acetate buffer and coupling buffer; E) third washing the mixture with Phosphate Buffered Solution; F) pouring the mixture into a chromatography column; G) allowing the mixture to settle; H) first passing, by gravity, a solution of saturated ammonium sulfate precipitated antibodies in loading buffer through the chromatography column; I) second passing, by gravity, loading buffer through the chromatography column until thoroughly washed; and J) second passing, by gravity, chromatography column elution buffer through the chromatography column which functions to remove and the purified antibodies. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7309690-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011098223-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005261194-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9427455-B2 |
| priorityDate | 1996-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.