| abstract |
A composition is provided, which comprises a purified cytoplasmic extract from eggs of Xenopus laevis which is capable of supporting activation of human sperm and complete replication of a human sperm genome. Methods of using the extract to achieve complete replication of the sperm genome are disclosed. A flow cytometric method for quantitatively monitoring replication of human sperm genomes is also disclosed. This method can be used for clinical assessment of sperm quality in a test sample of human sperm from an infertile male donor. |