| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e7aa001f3235c07b0298867908d45e21 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S424-812
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-82 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-1275 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-127 |
| filingDate |
1996-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1998-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75a83d7aed0b246432a32f05904777a0 |
| publicationDate |
1998-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5776770-A |
| titleOfInvention |
Isolation and uses of caveolae |
| abstract |
Methods of isolating and purifying caveolae, microdomains of GPI-anchored proteins, and membranes consisting essentially of caveolae associated with microdomains of GPI-anchored proteins from endothelial cell membranes are disclosed. The methods comprise coating a luminal surface of an endothelial cell membrane with an adherent first ionic material by perfusion from a luminal cavity adjacent to the endothelial cell membrane, forming a pellicle by contacting the first ionic material with a second ionic material, and isolating and purifying the pellicle. The pellicle is then processed to isolate the desired cellular component. Caveolae which are substantially free of microdomains of GPI-anchored proteins; microdomains of GPI-anchored proteins which are substantially free of caveolae; and membranes consisting essentially of caveolae, microdomains of GPI-anchored proteins, and caveolae associated with microdomains of GPI-anchored proteins; all of which are substantially free of other cellular elements, are also disclosed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6737516-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007160531-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2205264-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6903196-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8906390-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2008303057-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007275924-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7517968-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004115128-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2205264-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003199448-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005148762-A1 |
| priorityDate |
1995-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |