| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a333e6344b62fb21a7c1ee592b165fca |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2417
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-54 |
| filingDate |
1994-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1998-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7aec6cdeb14ca2dfc2fc3ce988c293d8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec9156dcb886894c943e4ebf0f0d944d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d99a3b956ef4928332f363b30509cfb3 |
| publicationDate |
1998-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5733723-A |
| titleOfInvention |
Stable gene amplification in chromosomal DNA of prokaryotic microorganisms |
| abstract |
Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable alpha -amylase, and mutants and variants of said strains. Preferred polypeptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the alpha -amylase encoding gene obtainable from B. licheniformis strain T5. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7842479-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6124097-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005227356-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007231841-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011020869-A1 |
| priorityDate |
1987-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |