| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d380910291671dc2738927a425603ad0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5094
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1996-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1997-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_681665fb1f7dfb8bb16d6a2ec060c40a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f601904c1f166becd38ef9ae3e40f9d |
| publicationDate |
1997-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5702884-A |
| titleOfInvention |
Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis |
| abstract |
Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9494579-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3167900-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010003700-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004106109-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010062415-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE43389-E
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5856145-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9291549-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007243542-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113755434-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102011080853-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8722347-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2557161-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8216797-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10436680-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10767084-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7262006-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7615346-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8067184-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8835127-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021002715-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9005989-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016252498-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2759307-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009280498-A1 |
| priorityDate |
1996-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |