| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d111d6dcb3d7a07e4e766163463ff9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-75 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4716
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-1019 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-475
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 |
| filingDate |
1995-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1997-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61c0661ea6379b54e925b552a343c8eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88c56a3ecbb95fa26a10dd3c6a905437
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e90b238b970433bf5a0f19f66c1bc77
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08d6b137ef3af804e272b183a9cb4f02 |
| publicationDate |
1997-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5656456-A |
| titleOfInvention |
Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
| abstract |
The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid alpha -carbon reactive group selected from the group consisting of N-terminal alpha -amine, C-terminal alpha -carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal alpha -amine, C-terminal alpha -carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid alpha -carbon reactive group. The unprotected terminal amino acid alpha -carbon reactive group is modified with at least one chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C-terminal amino acid of a recombinantly produced polypeptide. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7348423-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1094114-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003044420-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1362918-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003039659-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003219854-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7084256-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6787359-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003035807-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005191729-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5962270-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6410707-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9920759-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004058447-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008044903-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1094114-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6858410-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005058659-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6251635-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006018900-A1 |
| priorityDate |
1992-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |