abstract |
A sensitive assay method has been discovered that reduces the amount of non-specific binding present in an assay, the method comprising detecting an analyte present in a sample through a specific binding reaction in which either an analogue of the analyte or a specific binding partner of the analyte is immobilized on a solid-phase and said specific binding reaction produces a detectable product immobilized on said solid-phase that may be correlated to the amount of analyte present in the sample wherein said assay employs an effective amount of a surfactant selected from the group consisting of a polyoxyethylene-alkylether, a polyalkylene oxide-modified polydimethylsiloxane block copolymer, a polyalkylene oxide-modified polymethylsiloxane block copolymer, and mixtures thereof to reduce non-specific binding. |