| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a1012ab36c116b990c63cf6ac9aa5aa |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5418
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-475 |
| filingDate |
1993-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1997-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_590f0f2422ef439a9ebb6f30d0847657
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1eea43efed83d9125bac3de7351ffb4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e5a2659b5562ea7565630be6e3434a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dbf0c8d90c154b0cc5a4eed0f849aa4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28a60b7b553f1f539a9992929053ea54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bea97fe7df32c322fceac0f689fc0a09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c6a46ca68739a3e8fd7faf55065756a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80097a9e856bd2f484050f73f5c26cde |
| publicationDate |
1997-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5616485-A |
| titleOfInvention |
Streptomyces proteases and improved streptomyces strains for expression of peptides and polypeptides |
| abstract |
A family of proteases endogenous to Streptomyces cells degrade heterologous proteins secreted from Streptomyces host cells. The previously unidentified proteases include (1) tripeptidyl aminopeptidase-Streptomyces ("Tap"), (2) a Streptomyces protease ("Ssp") which displayed significant amino acid sequence homology to Subtilisin BPN' and showed an ability to remove tripeprides from the amino termini of proteins and peptides, and (3) other proteases derived from Streptomyces which degraded certain substrates under certain conditions. Degradation was alleviated by selective inhibition of secreted proteases or by using hosts with impaired capabilities to produce proteases. An irreversible inhibitor was designed based upon the mechanism and substrate specificity of the target protease. Hosts secreting high amounts of proteases were selected. Impaired hosts were produced by deleting or altering the nucleotide sequence for the proteases. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5821104-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6579698-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5989889-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6300117-B1 |
| priorityDate |
1993-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |