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publicationDate 1997-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5610036-A
titleOfInvention Mutant AOX2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
abstract A mutant AOX2 (alcohol oxidase 2) promoter derived from the natural AOX2 promoter by base sequence deletion, insertion, or substitution is disclosed. A microbial strain carrying a gene under the control of such a mutant AOX2 promoter can be obtained by growing in a methanol-containing medium a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter. A heterologous protein may be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from the mutant AOX2 promoter. A plasmid carrying the mutant AOX2 promoter is also provided.
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priorityDate 1991-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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