| abstract |
The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34+ stem and progenitor cells which can be used in human therapeutics. |