| abstract |
The invention provides methods for attaching detectable labels to the N 7 -formyl group, of imidazole ring-opened alkylated purines and purine derivatives, directly, through a linking group, or by subsequent derivatization of a blocking group. The invention provides simple and efficient methods for labeling polynucleotides. In addition, the invention provides derivatized ring-opened alkylated purines and purine derivatives, including reagents for chemical and enzymatic synthesis of detectably labeled polynucleotides. |